IMPROVEMENT IN THE HISTOCHEMICAL LOCALIZATION OF SUCCINIC DEHYDROGENASE BY USE OF NITRONEOTETRAZOLIUM CHLORIDE | Zendy

Bjarne Pearson | Zendy

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IMPROVEMENT IN THE HISTOCHEMICAL LOCALIZATION OF SUCCINIC DEHYDROGENASE BY USE OF NITRONEOTETRAZOLIUM CHLORIDE

Author(s) -

Bjarne Pearson

Publication year - 1958

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/6.2.112

Subject(s) - formazan , tetrazolium chloride , chemistry , dimethylformamide , incubation , succinic dehydrogenase , chloride , dehydrogenase , enzyme , biochemistry , chromatography , organic chemistry , medicine , ischemia , solvent

A new method for the histochemical localization of succinic dehydrogenase activity has been described in which the resultant formazan is free of crystal formation. The resultant formazan is extremely fine and granular permitting accurate and precise localization of the enzyme. The tetrazolium salt used was 2,2'-diphenyl-5,5' - di - ( m - nitrophenyl) - 3,3' - (4,4' - biphenylene) ditetrazolium chloride. Minimal granular artifacts which appear may be eliminated by dissolving the tetrazolium salt in N,N-dimethylformamide and rinsing the slide in 80% alcohol following incubation. This has a tendency to dissolve fat and prevent aggregation of granular artifacts which may appear subsequently upon storage of slides.

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