Open AccessDETERMINATION OF PROTEIN AND LIPID LOST DURING OSMIC ACID FIXATION OF TISSUES AND CELLULAR PARTICULATES
Author(s) -
R. Duncan Dallam,
Ruth Curtiss Telfer
Publication year - 1957
Publication title -
journal of histochemistry and cytochemistry/the journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/5.2.178
Subject(s) - fixative , microsome , chemistry , electron microscope , mitochondrion , fixation (population genetics) , biochemistry , cytoplasm , in vitro , physics , optics , gene
Heart and kidney fragments and liver mitochondria and microsomes were prepared for electron microscopy by standard methods. The fixative, dehydration and embedding mediums each were analyzed separately for protein and lipid removed. It was demonstrated that protein and lipid were lost from mitochondria and microsomes and that protein was lost from tissue fragments during their preparation for electron microscopic examination. The significance of these results with respect to investigations concerned with the correlation of function to morphology is discussed.