HISTOCHEMICAL DEMONSTRATION OF AMINOPEPTIDASE IN NORMAL AND PATHOLOGICAL SKIN

\v(, umuudcm’t ook mu mstnniber of experiments ss’it h the hsistochemicmil unet hum! for densonsst rmut ion of mmniuin5o )ept i(!mise mu(’con(Iinsg to Bttrstonse arid Folk (J. Histochmem. mu. Cytochern. 4: 217 (l956 ins usomusimul arid )muthuological skin. Inspressiomsable pictunres we obtained not. only ins fro7ems-dnied mmutenimul, hunt inn fresh frozen sections. The time of incuibmution is less than ins frozen-dried nssmitenial. However, cnystal!izmution of the red azo-dyestuff occurs after muboumi 20-3() nssinsumtes. ‘[‘hue specificity of the hist.ochemicmul muminsopeptidmuse technic with tnse of m -leunc ’l-fl-nsaphthvlmumide as sutl)strate is funnthenmone certaims, mis in m’its’o tru’psini mmmd I)(’l)sims mune riot muble to hivdnolvse this subst rate. Besides mi mnodermute mumninsopeptidmise-activitv ins the lower lmiyers of stratum Nlalpighi of I Ime s ki mu t lucre is mu st rang posit ive react ions i mu t hue eccninse swemut glands arid mi less remict ions in (‘mipillmiries. \‘eny nensimurkable is thse high enuzyme activity ins the epidermis in dermatitis munm(Ieczemusmm junst below’ the vesicles. It is sumggested, that these resunlts are ins direct relation with tIme forunmutions of the vesicles ins these dermatoses. The resunlts in skin tunmors are also