Comparison of LR White and Unicryl as embedding media for light and electron immunomicroscopy of chromaffin cells. | Zendy

G. Goping | Zendy; Gemma A.J. Kuijpers | Zendy; Raúl Vinet | Zendy; Harvey B. Pollard | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Comparison of LR White and Unicryl as embedding media for light and electron immunomicroscopy of chromaffin cells.

Author(s) -

G. Goping,

Gemma A.J. Kuijpers,

Raúl Vinet,

Harvey B. Pollard

Publication year - 1996

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/44.3.8648090

Subject(s) - immunogold labelling , ultrastructure , tyrosine hydroxylase , osmium tetroxide , electron microscope , chemistry , microbiology and biotechnology , biology , biochemistry , enzyme , anatomy , physics , optics

LR White and Unicryl are members of the same family of acrylic embedding resins and are very suitable for "on grid" postembedding immunogold labeling. We studied the ultrastructure of LR White- and Unicryl-embedded cultured chromaffin cells and the immunolocalization of three chromaffin cell proteins, the enzymes dopamine-beta-hydroxylase (DbetaH) and tyrosine hydroxylase (TH), and the membrane fusion and Ca2+ channel protein synexin (annexin VII). We report here that Unicryl is preferable to LR White as an embedding medium for electron microscopy when osmium tetroxide fixation is omitted. The basis for this distinction is better ultrastructural preservation and improved immunodetection efficiency.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research