LacZ staining in paraffin-embedded tissue sections. | Zendy

Petrus J. Hendrikx | Zendy; Jessica Vermeulen | Zendy; Anton Hagenbeek | Zendy; Marcel Vermey | Zendy; Anton C. Martens | Zendy

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LacZ staining in paraffin-embedded tissue sections.

Author(s) -

Petrus J. Hendrikx,

Jessica Vermeulen,

Anton Hagenbeek,

Marcel Vermey,

Anton C. Martens

Publication year - 1996

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/44.11.8918907

Subject(s) - immunostaining , microbiology and biotechnology , acute promyelocytic leukemia , staining , biotinylation , chemistry , pathology , biology , immunohistochemistry , biochemistry , medicine , gene , retinoic acid

Femora and tibiae of rats carrying leukemia from a LacZ-marked acute promyelocytic leukemia-derived leukemic cell line (LT12NL15) were decalcified using EDTA and routinely embedded in paraffin. Sections were used to develop for the first time an immunostaining method for LacZ, employing catalyzed reporter deposition (CARD) based on the deposition of biotinylated tyramine. This method was used to study homing and adhesion of leukemic cells.

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