A simplified method for obtaining 0.5-microns sections of small tissue specimens embedded in PEG. | Zendy

K A Holtham | Zendy; Norma Slepecky | Zendy

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A simplified method for obtaining 0.5-microns sections of small tissue specimens embedded in PEG.

Author(s) -

K A Holtham,

Norma Slepecky

Publication year - 1995

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/43.6.7769235

Subject(s) - araldite , peg ratio , polyethylene glycol , agarose , materials science , staining , polymer , fixation (population genetics) , biomedical engineering , biophysics , chemistry , composite material , chromatography , adhesive , pathology , biology , biochemistry , finance , layer (electronics) , economics , gene , medicine

We describe a new method for embedding small specimens in polyethylene glycol (PEG) 4000. This method preserves cell morphology and provides sensitive immunocytochemical labeling with excellent subcellular resolution. Small tissues are embedded in agarose so that they can be grouped together and oriented for sectioning before infiltration with PEG 4000, a water-soluble polymer. Fixation, embedding, sectioning, and staining can be performed in 1 day. Results from immunocytochemical studies localizing actin and tubulin on 0.5-micron sections of PEG-embedded specimens are compared with those obtained on semi-thin sections of araldite-embedded specimens and demonstrate the ease, speed, and increased sensitivity of this embedding method.

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