A protein-specific monoclonal antibody to rat liver beta 1-->4 galactosyltransferase and its application to immunohistochemistry. | Zendy

Junichi KAWANO | Zendy; Shinsuke Ide | Zendy; Tsutomu Oinuma | Zendy; Tatsuo Suganuma | Zendy

Open Access

A protein-specific monoclonal antibody to rat liver beta 1-->4 galactosyltransferase and its application to immunohistochemistry.

Author(s) -

Junichi KAWANO,

Shinsuke Ide,

Tsutomu Oinuma,

Tatsuo Suganuma

Publication year - 1994

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/42.3.8308253

Subject(s) - galactosyltransferase , immunohistochemistry , monoclonal antibody , golgi apparatus , immunoelectron microscopy , microbiology and biotechnology , staining , sublingual gland , biology , antibody , epididymis , antigen , chemistry , submandibular gland , biochemistry , endocrinology , immunology , enzyme , endoplasmic reticulum , genetics , botany , sperm

We have produced a new protein-specific monoclonal antibody (MAb) to rat liver beta 1-->4 galactosyltransferase. This MAb, GTL2, was selected as the most reactive IgG to a periodate-treated antigen. Antigen and protein specificities of GTL2 were verified by immunoblotting of a non-glycosylated recombinant protein of human galactosyltransferase and enzymatically deglycosylated rat galactosyltransferase. Using GTL2, an immunohistochemical study was done in rat liver, epididymis, and salivary glands. Intense staining was observed in Golgi areas of epididymal duct epithelial cells, and submandibular and sublingual acinar cells. Hepatocytes showed weaker staining. Immunoelectron microscopic observation revealed that the staining was exclusively localized in trans-Golgi membranes of these cells.

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