Non-isotopic in situ hybridization method for mitochondria in oncocytes. | Zendy

Vijay Varma | Zendy; C M Cerjan | Zendy; Karen L. Abbott | Zendy; Susan B. Hunter | Zendy

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Non-isotopic in situ hybridization method for mitochondria in oncocytes.

Author(s) -

Vijay Varma,

C M Cerjan,

Karen L. Abbott,

Susan B. Hunter

Publication year - 1994

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/42.2.8288868

Subject(s) - digoxigenin , mitochondrion , microbiology and biotechnology , in situ hybridization , in situ , mitochondrial dna , biology , oligonucleotide , dna , polymerase chain reaction , biotin , hybridization probe , complementary sequences , chemistry , biochemistry , messenger rna , gene , statistics , mathematics , organic chemistry

We used in situ hybridization to specifically identify mitochondria in a series of formalin-fixed, paraffin-embedded oncocytic lesions. Digoxigenin-labeled DNA probes were generated by the polymerase chain reaction (PCR), with primers designed to amplify a mitochondrion-specific 154 BP sequence within the ND4 coding region. Probes were hybridized with mitochondrial DNA under stringent conditions. Oncocytes were strongly and consistently stained, reflecting the high copy number of mitochondrial DNA within these cells. Because of the presence of endogenous biotin within mitochondria, digoxigenin is preferable to biotin as a label for detection of mitochondria.

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