An alternative method for preparation of Schiff-like reagent from osmium-ammine complex for selective staining of DNA on thin Lowicryl sections. | Zendy

Valya T. Mikhaylova | Zendy; D Markov | Zendy

Open Access

An alternative method for preparation of Schiff-like reagent from osmium-ammine complex for selective staining of DNA on thin Lowicryl sections.

Author(s) -

Valya T. Mikhaylova,

D Markov

Publication year - 1994

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/42.12.7983365

Subject(s) - staining , osmium , feulgen stain , reagent , stain , chemistry , osmium tetroxide , periodic acid–schiff stain , chromatography , schiff base , dna , biochemistry , organic chemistry , stereochemistry , biology , catalysis , optics , genetics , electron microscope , physics , ruthenium

Batches of osmium-ammine (OA) complex vary considerably in staining properties when used in a Feulgen-like reaction for selective staining of DNA-containing structures. An alternative procedure for preparation of Schiff-like reagent from OA complex is described. It is based on generation of H2SO3, respectively SO2, within the OA solution and ensures more favorable conditions for production of a Schiff-like stain than bubbling with SO2 does. The method is reproducible and yields high staining intensity. Data obtained suggest that the ability of OA complex to produce Feulgen-like staining is strongly influenced by variations in its chemical composition. Their unfavorable effect can be overcome by selecting suitable conditions for preparation of a Schiff-like reagent. Conditions for obtaining specific and sensitive Feulgen-like staining are determined.

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