Establishment of a novel monoclonal antibody, SE-1, which specifically reacts with rat hepatic sinusoidal endothelial cells. | Zendy

Tosei Ohmura | Zendy; Katsuhiko Enomoto | Zendy; Hiroshi Satoh | Zendy; N Sawada | Zendy; Makoto Mori | Zendy

Open Access

Establishment of a novel monoclonal antibody, SE-1, which specifically reacts with rat hepatic sinusoidal endothelial cells.

Author(s) -

Tosei Ohmura,

Katsuhiko Enomoto,

Hiroshi Satoh,

N Sawada,

Makoto Mori

Publication year - 1993

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/41.8.8331290

Subject(s) - immunogen , immunoelectron microscopy , monoclonal antibody , antigen , antibody , biology , microbiology and biotechnology , chemistry , pathology , immunology , medicine

A monoclonal antibody, SE-1, which specifically reacted with rat hepatic sinusoidal endothelial cells (HSE), was established with elutriator-separated HSE as immunogen. No crossreactivity was observed with other types of endothelial cells or non-endothelial cells. By immunoelectron microscopy, the antigen recognized by MAb SE-1 was localized to the membrane surface of HSE. Immunoblot analysis revealed that SE-1 recognized a single 45 KD band. Although the SE-1 antigen is not yet characterized, the above evidence strongly suggests that it is related to the specific function of HSE. Therefore, SE-1 may be a useful marker to study the role of HSE in various pathophysiological conditions of the liver.

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