Comparison of the sensitivities of ribonucleic acid and oligonucleotide probes for in situ detection of Theiler's virus mRNA. | Zendy

C Aubert | Zendy; S Ozden | Zendy

Open Access

Comparison of the sensitivities of ribonucleic acid and oligonucleotide probes for in situ detection of Theiler's virus mRNA.

Author(s) -

C Aubert,

S Ozden

Publication year - 1993

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/41.7.8515052

Subject(s) - riboprobe , oligonucleotide , oligomer restriction , microbiology and biotechnology , in situ , rna , in situ hybridization , molecular probe , chemistry , nucleotide , hybridization probe , nucleic acid thermodynamics , sense (electronics) , biology , messenger rna , virology , dna , biochemistry , gene , organic chemistry

To evaluate the sensitivity of in situ detection of the Theiler's virus genome, we hybridized BHK-21-infected cells with antisense ribo- and oligonucleotide 35S-labeled probes. The sensitivity achieved with the anti-sense 280-nucleotide riboprobe was similar to that obtained with a 93-mer oligonucleotide probe. However, more reproducible and accurate results were obtained with the riboprobe. With long exposure times, the background was higher with the oligonucleotide probe than with the RNA probe. The background was improved by using freshly labeled oligonucleotide probe.

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