Combined immunocytochemistry and enzyme cytochemistry on ultra-thin cryosections: a new method. | Zendy

Toshihiro Takizawa | Zendy; John M. Robinson | Zendy

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Combined immunocytochemistry and enzyme cytochemistry on ultra-thin cryosections: a new method.

Author(s) -

Toshihiro Takizawa,

John M. Robinson

Publication year - 1993

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/41.11.8409371

Subject(s) - cytochemistry , immunocytochemistry , enzyme , immunogold labelling , chemistry , biology , biochemistry , pathology , antibody , medicine , immunology

We combined immunocytochemistry and enzyme cytochemistry to localize two different proteins on the same ultrathin cryosection. In this method the immunocytochemical localization is visualized with colloidal gold probes and the enzyme cytochemical detection is achieved with cerium as the capture agent. The immunocytochemistry is conducted first so that any potential adverse effects of the enzyme cytochemical procedure will not alter the antibody binding properties of the cryosections.

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