Open AccessA specific fluorogenic reaction for tryptophan residues using isatin in organic solvents.
Author(s) -
Clara Isabel Trigoso,
N Ibañez,
Juan C. Stockert
Publication year - 1993
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/41.10.8245414
Subject(s) - isatin , tryptophan , chemistry , performic acid , fluorescence , acetone , ethanol , cyanine , organic chemistry , biochemistry , amino acid , physics , quantum mechanics
The development of new fluorogenic reactions for specific chemical groups is of increasing interest in cytochemistry. We describe the application of the nonfluorescent compound isatin on methanol-fixed blood smears. When treated with 0.1% isatin in absolute ethanol or acetone for 30 min and observed under violet-blue exciting light, eosinophil leucocyte granules show a bright green fluorescence. This fluorogenic reaction is abolished after blocking of tryptophan residues by performic acid oxidation. Spectrofluorometric studies with amino acids in vitro reveal that isatin forms a unique and specific fluorescent product with tryptophan.
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