In situ hybridization of bone matrix proteins in undecalcified adult rat bone sections. | Zendy

Tohru Ikeda | Zendy; Shogo Nomura | Zendy; Akira Yamaguchi | Zendy; Takeshi Suda | Zendy; Shusaku Yoshiki | Zendy

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In situ hybridization of bone matrix proteins in undecalcified adult rat bone sections.

Author(s) -

Tohru Ikeda,

Shogo Nomura,

Akira Yamaguchi,

Takeshi Suda,

Shusaku Yoshiki

Publication year - 1992

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/40.8.1619274

Subject(s) - osteopontin , osteocalcin , in situ hybridization , matrix gla protein , bone resorption , chemistry , matrix (chemical analysis) , bone cell , endocrinology , resorption , messenger rna , bone remodeling , medicine , alkaline phosphatase , biology , pathology , biochemistry , gene , kidney disease , hyperphosphatemia , chromatography , enzyme

We have developed a method for in situ hybridization of adult bone tissue utilizing undecalcified sections and have used it to histologically examine the mRNA expression of non-collagenous bone matrix proteins such as osteocalcin (bone Gla protein, BGP), matrix Gla protein (MGP), and osteopontin in adult rats. Expression was compared with that in bone tissues of newborn rats. In the adult bone tissue, osteocalcin mRNA was strongly expressed in periosteal and endosteal cuboidal osteoblasts but not in primary spongiosa near the growth plate. Osteopontin mRNA was strongly expressed in cells present on the bone resorption surface, osteocytes, and hypertrophic chondrocytes, but not in cuboidal osteoblasts on the formation surface. Osteopontin and osteocalcin mRNAs were expressed independently and the distribution of cells expressing osteopontin mRNA corresponded with acid phosphatase-positive mononuclear cells and osteoclasts. Expression of MGP mRNA was noted only in hypertrophic chondrocytes. In newborn rat bone tissues, expression of osteocalcin mRNA was much weaker than in adult rat bone tissues. These results clearly indicate the differential expression of mRNAs of non-collagenous bone matrix proteins in adult rat bone tissues.

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