Open AccessLight and electron microscopic studies of cellular localization of oPL with monoclonal and polyclonal antibodies.
Author(s) -
F. B. P. Wooding,
Gareth J. Morgan,
I. A. Forsyth,
Geoffrey W. Butcher,
Amanda Hutchings,
S. A. Billingsley,
P. D. Gluckman
Publication year - 1992
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/40.7.1607634
Subject(s) - polyclonal antibodies , monoclonal antibody , immunogold labelling , immunocytochemistry , placental lactogen , syncytium , golgi apparatus , biology , electron microscope , antibody , monoclonal , microbiology and biotechnology , human placental lactogen , immunohistochemistry , cytoplasm , pathology , placenta , cell , immunology , biochemistry , endocrinology , endoplasmic reticulum , fetus , pregnancy , medicine , genetics , physics , optics
Accurate knowledge of placental lactogen localization is fundamental to any hypothesis of its synthesis and secretion. We used locally generated monoclonal and polyclonal antibodies from three separate sources to localize ovine placental lactogen immunoreactivity on light and electron microscope Lowicryl K4M sections of ovine placentomes of 97-145 days of gestation, using immunogold techniques. All antibodies demonstrated that immunoreactivity was exclusively localized in the trophoectoderm binucleate cell Golgi body and granules and in granules in the syncytium derived from binucleate cell migration. No evidence was found to support a recent claim that monoclonal antibodies to oPL that were produced in Canada indicated a predominant localization of ovine placental lactogen to uninucleate trophectodermal cells.
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