The use of osmicated tissues for Lowicryl K4M embedding. | Zendy

Antonio Nanci | Zendy; M.R. Mazariegos | Zendy; Micheline Fortin | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

The use of osmicated tissues for Lowicryl K4M embedding.

Author(s) -

Antonio Nanci,

M.R. Mazariegos,

Micheline Fortin

Publication year - 1992

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/40.6.1588031

Subject(s) - glutaraldehyde , cytochemistry , fixative , osmium tetroxide , chemistry , ultrastructure , anatomy , biology , biochemistry , electron microscope , chromatography , cytoplasm , physics , optics

Tissue chopper slices of rat incisor, rat parotid gland, and chicken tibiae, fixed with 1% glutaraldehyde, were post-fixed with potassium ferrocyanide-reduced osmium tetroxide, dehydrated with methanol, and conventionally embedded in Lowicryl K4M at -20 degrees C. The tissues showed an ultrastructural appearance comparable with that of their Epon-embedded counterparts and, in particular, the Golgi apparatus was well defined. Furthermore, Lowicryl K4M-embedded osmicated tissues permitted both post-embedding lectin-gold cytochemistry and immunogold labeling.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research