A 1.4-nm gold cluster covalently attached to antibodies improves immunolabeling. | Zendy

James F. Hainfeld | Zendy; F. Furuya | Zendy

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A 1.4-nm gold cluster covalently attached to antibodies improves immunolabeling.

Author(s) -

James F. Hainfeld,

F. Furuya

Publication year - 1992

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/40.2.1552162

Subject(s) - immunolabeling , covalent bond , gold cluster , colloidal gold , electron microscope , nanotechnology , chemistry , immunoconjugate , surface plasmon resonance , microscopy , resolution (logic) , materials science , crystallography , antibody , optics , nanoparticle , biology , monoclonal antibody , physics , immunology , electronic structure , immunohistochemistry , computational chemistry , organic chemistry , artificial intelligence , computer science

A large gold cluster (Au1.4nm) was covalently coupled to IgG and Fab' fragments. Its gold core is 1.4 nm in diameter and the Fab'-Au1.4nm immunoconjugate is the smallest gold immunoprobe that can be seen directly in the conventional electron microscope. It is useful in high-resolution immunolabeling, providing a resolution of 7.0 nm. The cluster's visibility can be enhanced with silver development for use in EM or light microscopy for histological purposes, or to detect less than or equal to 0.2 pg of antigen in immunoblots. By using a gold compound with covalent attachment, a number of advantages over colloidal gold probes are realized, including better resolution, stability, uniformity, sensitivity, and complete absence of aggregation; its small size should also improve penetration and more quantitative labeling of antigenic sites.

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