Co-localization of an endocytic marker and acid phosphatase in a tubular/reticular compartment in macrophages. | Zendy

Zhiyong Luo | Zendy; Jeffrey Robinson | Zendy

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Co-localization of an endocytic marker and acid phosphatase in a tubular/reticular compartment in macrophages.

Author(s) -

Zhiyong Luo,

Jeffrey Robinson

Publication year - 1992

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/40.1.1729356

Subject(s) - lucifer yellow , reticular connective tissue , compartment (ship) , acid phosphatase , endocytic cycle , reticular cell , microbiology and biotechnology , chemistry , phosphatase , biochemistry , biology , enzyme , cell , anatomy , endocytosis , intracellular , immunology , spleen , geology , oceanography , gap junction

Cultured resident murine macrophages are incubated in the continuous presence of the fluorescent endocytic marker Lucifer Yellow and a phorbol ester that activates protein kinase C. Under these steady-state labeling conditions the fluorescent tracer was, for the most part, in a tubular/reticular compartment. Enzyme cytochemical localization of acid phosphatase in the same cells showed essentially a one-to-one correlation between the Lucifer Yellow- and acid phosphatase-containing compartments. Procedures for epifluorescence observation and subsequent enzyme cytochemical examination of the same whole mount cells are described. In addition, chemical fixation methods for the preservation of this labile tubular/reticular compartment are presented.

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