TWO TYPES OF ACIDOPHILS IN THE ANTERIOR PITUITARY OF THE FROG AND THEIR RELATION TO THE PERIODIC ACID SCHIFF REACTION | Zendy

Robert Ortman | Zendy

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TWO TYPES OF ACIDOPHILS IN THE ANTERIOR PITUITARY OF THE FROG AND THEIR RELATION TO THE PERIODIC ACID SCHIFF REACTION

Author(s) -

Robert Ortman

Publication year - 1956

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/4.5.471

Subject(s) - periodic acid–schiff stain , orange g , cytoplasm , glycogen , anterior pituitary , biochemistry , chemistry , aniline , biology , staining , organic chemistry , catalysis , hormone , genetics

Koneff's ('38) modification of Mallory-azan reveals the presence of azocarmine, orange G, aniline blue, and chromophobic cells in the pars distalis of the frog. A strong periodic acid Schiff (PAS) reaction, apparently of carbohydrate-containing material, uniformly distributed throughout the cytoplasm, is found in the azocarmine cells and in the aniline blue cells. Certain of the orange G cells contained PAS-positive material confined to cytoplasmic vesicles. The PAS-positive material is probably not glycogen. It was not possible to distinguish between the various PAS-reactive sites with the cytochemical methods employed in this study.

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