Localization of carbonic anhydrase in identified glial cells of the leech central nervous system: application of histochemical and immunocytochemical methods. | Zendy

Barbara Riehl | Zendy; W. R. Schlue | Zendy

Open Access

Localization of carbonic anhydrase in identified glial cells of the leech central nervous system: application of histochemical and immunocytochemical methods.

Author(s) -

Barbara Riehl,

W. R. Schlue

Publication year - 1990

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/38.8.2114440

Subject(s) - hirudo medicinalis , leech , neuropil , carbonic anhydrase , immunofluorescence , biology , central nervous system , immunocytochemistry , neuron , biochemistry , neuroglia , cytochemistry , enzyme , chemistry , microbiology and biotechnology , antibody , neuroscience , immunology , world wide web , computer science , endocrinology

We localized the enzyme carbonic anhydrase (CA) in frozen sections of the leech (Hirudo medicinalis) central nervous system by two histochemical techniques and the indirect immunofluorescence technique. Hansson's cobalt precipitation method and the use of 1-dimethylamino-naphthalene-5-sulfonamide (DNSA) to build a fluorescent enzyme-substrate complex showed that glial cells are the sites of CA activity in the leech. Neuropil and connective glial cells surrounding the axons had strong CA activity, whereas packet glial cells, which surround neuron cell bodies, and neurons themselves remained unstained. Glial cells reacted markedly with FITC-coupled antibodies against CA isoenzyme II, but experiments with antibodies against CA isoenzyme I showed no reaction.

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