Open AccessIontophoretic injection of fluoro-gold and other fluorescent tracers.
Author(s) -
L C Schmued,
Lennart Heimer
Publication year - 1990
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/38.5.2332627
Subject(s) - iontophoresis , fluorescence , propidium iodide , chemistry , biophysics , optics , biochemistry , medicine , biology , radiology , physics , apoptosis , programmed cell death
Fluoro-gold, a retrograde axonally transported fluorescent tracer, has been successfully iontophoresed into brain regions to yield small injection sites with no detectable dye along the track. Previous difficulties involving iontophoretic application of fluoro-gold were overcome through the use of a cacodylate vehicle. This vehicle was also found to be suitable for iontophoresing other fluorescent tracers, such as propidium iodide or Fast Blue.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom