Localization of cerium-based reaction products by scanning laser reflectance confocal microscopy. | Zendy

John M. Robinson | Zendy; Bruce E. Batten | Zendy

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Localization of cerium-based reaction products by scanning laser reflectance confocal microscopy.

Author(s) -

John M. Robinson,

Bruce E. Batten

Publication year - 1990

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/38.3.2406336

Subject(s) - cerium , confocal , cytochemistry , hydrogen peroxide , microscope , microscopy , scanning electron microscope , confocal microscopy , laser , confocal laser scanning microscopy , materials science , acid phosphatase , chemistry , optics , biophysics , inorganic chemistry , biochemistry , enzyme , biology , physics , composite material

Scanning laser confocal microscopy was utilized to visualize sites of hydrogen peroxide release from stimulated neutrophils and lysosomal acid phosphatase in these and other cells using cerium in the detection systems. Imaging of the cerium-containing reactions was achieved by employing the reflectance mode of this instrument. Localization of these products at the light microscope level was direct and did not require other reactions to generate a visible product. This new approach to cerium cytochemistry should prove useful for many applications.

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