Immuno- and affinity probes for electron microscopy: a review of labeling and preparation techniques. | Zendy

John W. Stirling | Zendy

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Immuno- and affinity probes for electron microscopy: a review of labeling and preparation techniques.

Author(s) -

John W. Stirling

Publication year - 1990

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/38.2.2405054

Subject(s) - cryofixation , immunogold labelling , transmission electron microscopy , nanotechnology , microscopy , electron microscope , biophysics , chemistry , computational biology , computer science , materials science , biology , pathology , physics , optics , medicine

Immuno- and affinity probes are widely used in biology and medicine, and are becoming essential tools for the elucidation of cell structure and function. This article reviews and discusses the bewildering array of probes and preparation techniques now available for the investigation of sectioned material by transmission electron microscopy, with critical analysis of their merits. Emphasis is placed on immunogold probes and methods useful for routine preparation, gathering together information that may be used to improve labeling techniques. New data on inert dehydration for the localization of sensitive epitopes without chemical or cryofixation is presented.

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