Electron microscopy of gold-labeled human and equine chromosomes. | Zendy

Paul-Emil Messier | Zendy; Régen Drouin | Zendy; C.-L. Richer | Zendy

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Electron microscopy of gold-labeled human and equine chromosomes.

Author(s) -

Paul-Emil Messier,

Régen Drouin,

C.-L. Richer

Publication year - 1989

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/37.9.2768813

Subject(s) - metaphase , sister chromatids , immunogold labelling , biology , microbiology and biotechnology , chromatid , karyotype , chromosome , prophase , chromatin , mitosis , electron microscope , dna , genetics , meiosis , antibody , optics , gene , physics

We present an immunochemical technique for the detection of 5-bromo-2'-deoxyuridine (BrdU) incorporated discontinuously into the chromosomal DNA. A monoclonal anti-BrdU antibody and a protein A-gold complex were used to produce chromosome banding of human and equine chromosomes, specific for electron microscopy (EM). Well-defined bands, symmetry of sister chromatids, concordance between homologues, and band patterns similar to those observed by light microscopy facilitate chromosome identification and karyotyping. From prophase to late metaphase, chromosomes condense and bands appear to fuse. The fusion appears to be owing to chromatin reorganization. Our results underline the value of using immunogold reagents, which are ideal probes for antigen localization on chromosomes.

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