Enzyme histochemical demonstration of alkaline phosphatase activity in plastic-embedded tissues using a Gomori-based cerium-DAB technique. | Zendy

Harry van Goor | Zendy; P O Gerrits | Zendy; Mj Hardonk | Zendy

Open Access

Enzyme histochemical demonstration of alkaline phosphatase activity in plastic-embedded tissues using a Gomori-based cerium-DAB technique.

Author(s) -

Harry van Goor,

P O Gerrits,

Mj Hardonk

Publication year - 1989

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/37.3.2465338

Subject(s) - cerium , alkaline phosphatase , chemistry , acetone , calcium , phosphate , nuclear chemistry , enzyme , biochemistry , inorganic chemistry , organic chemistry

We describe a new method for light microscopic demonstration of alkaline phosphatase (ALP) activity in plastic-embedded sections. Rat tissues were fixed in acetone (-20 degrees C), infiltrated in glycol methacrylate (GMA), and embedded at 0 degrees C. Sections were cut at 1 and 2 microns, dried at room temperature, and incubated in the conventional Gomori medium. Cerium chloride was used to convert calcium phosphate into cerium phosphate, which was subsequently converted into cerium perhydroxide. The slight yellow precipitate of cerium perhydroxide was amplified using 3,3'-diaminobenzidine tetrahydrochloride (DAB). For comparison, tissue sections were processed according to the calcium-cobalt method. The method described combines exact localization of ALP activity with optimal preservation of tissue morphology.

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