Synthesis of a more stable osmium ammine electron-dense DNA stain. | Zendy

Ada L. Olins | Zendy; Bruce A. Moyer | Zendy; S H Kim | Zendy; David P. Allison | Zendy

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Synthesis of a more stable osmium ammine electron-dense DNA stain.

Author(s) -

Ada L. Olins,

Bruce A. Moyer,

S H Kim,

David P. Allison

Publication year - 1989

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/37.3.2465337

Subject(s) - osmium , stain , reagent , staining , dna , chemistry , negative stain , osmium tetroxide , biophysics , biochemistry , electron microscope , biology , organic chemistry , catalysis , ruthenium , optics , genetics , physics

Specific DNA staining for electron microscopic observation is simplified by a shorter synthesis of the staining reagent. The new, more reliable reagent, osmium ammine-B, is stable for more than a year, dissolves completely in water, and does not require reoptimization of staining conditions for every batch, yet reproducibly gives strong contrast to DNA-containing structures.

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