Rapid low molecular weight polyethylene glycol embedding protocol for immunocytochemistry. | Zendy

J Mowery | Zendy; J Chesner | Zendy; S Spangenberger | Zendy; Douglas C. Hixson | Zendy

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Rapid low molecular weight polyethylene glycol embedding protocol for immunocytochemistry.

Author(s) -

J Mowery,

J Chesner,

S Spangenberger,

Douglas C. Hixson

Publication year - 1989

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/37.10.2674274

Subject(s) - polyethylene glycol , peg ratio , antigenicity , agarose , immunocytochemistry , immunoperoxidase , chemistry , membrane , scanning electron microscope , electron microscope , materials science , biophysics , biomedical engineering , pathology , monoclonal antibody , chromatography , biochemistry , antigen , antibody , biology , immunology , medicine , composite material , physics , optics , finance , economics

We describe an alternative polyethylene glycol (PEG) embedding procedure which utilizes PEG 200 for dehydration and PEG 600 for infiltration and embedding of perfusion-fixed rat liver. PEG 600 has a melting point of 22 degrees C, enabling infiltration of fixed tissue to be performed at room temperature. Sections (2 microM) cut in a cryostat at -20 degrees C and immobilized in agarose were readily labeled by immunoperoxidase protocols with monoclonal antibodies to hepatocyte membrane antigens. Subsequent examination by light microscopy or by electron microscopy after re-embedding in resin and ultra-thin sectioning showed excellent preservation of morphology, with minimal impairment of antigenicity.

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