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We developed two monoclonal antibodies, E5 and F5, which react with mouse epidermal growth factor (mEGF) and urogastrone. These antibodies and a rabbit antiserum to mEGF were used for immunohistochemical analysis of staining reactions in rodent and human tissues. Our results do not confirm the published reports of EGF-like immunoreactivity in Brunner's glands, human submandibular glands, human kidney tissue, or rodent brain sections.

An immunohistochemical study performed with monoclonal and polyclonal antibodies to mouse epidermal growth factor.