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An immunohistochemical study performed with monoclonal and polyclonal antibodies to mouse epidermal growth factor.
Author(s) -
H J Beerstecher,
C Huiskens-van der Meij,
S. O. Warnaar
Publication year - 1988
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/36.9.3261310
Subject(s) - polyclonal antibodies , immunohistochemistry , monoclonal antibody , antiserum , epidermal growth factor , antibody , biology , pathology , monoclonal , staining , microbiology and biotechnology , medicine , immunology , cell culture , genetics
We developed two monoclonal antibodies, E5 and F5, which react with mouse epidermal growth factor (mEGF) and urogastrone. These antibodies and a rabbit antiserum to mEGF were used for immunohistochemical analysis of staining reactions in rodent and human tissues. Our results do not confirm the published reports of EGF-like immunoreactivity in Brunner's glands, human submandibular glands, human kidney tissue, or rodent brain sections.

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