Immunocytochemical identification of bovine Langerhans cells by use of a monoclonal antibody directed against class II MHC antigens. | Zendy

Lorraine Bryan | Zendy; Philip Griebel | Zendy; Deborah M. Haines | Zendy; William C. Davis | Zendy; John R. Allen | Zendy

Open Access

Immunocytochemical identification of bovine Langerhans cells by use of a monoclonal antibody directed against class II MHC antigens.

Author(s) -

Lorraine Bryan,

Philip Griebel,

Deborah M. Haines,

William C. Davis,

John R. Allen

Publication year - 1988

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/36.8.3292647

Subject(s) - monoclonal antibody , antigen , epidermis (zoology) , immunocytochemistry , microbiology and biotechnology , immunohistochemistry , biology , mhc class i , antibody , bovine serum albumin , primary and secondary antibodies , major histocompatibility complex , chemistry , immunology , anatomy , endocrinology

We undertook a study to develop a reliable light microscopic technique for identifying Langerhans cells (LC) in bovine epidermis. Monoclonal antibodies (MCA) detecting bovine class II MHC antigens were used in conjunction with an avidin-biotin-peroxidase complex (ABC) immunocytochemical staining method. The specificity of the MCA for LC was confirmed ultrastructurally by use of gold-labeled second antibody. Epidermal sheets and epidermal single-cell suspensions examined by light microscopy confirm that bovine epidermal LC express class II antigens. Anti-bovine class II MCA is a dependable reagent for identification of LC in normal bovine epidermis.

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