Quantitative histochemical determination of Na+ and K+ in microscopic samples using carbon furnace atomic absorption spectrometry. | Zendy

Ilene Sussman | Zendy; Leslie C. MacGregor | Zendy; Barry R. Masters | Zendy; F M Matschinsky | Zendy

Open Access

Quantitative histochemical determination of Na+ and K+ in microscopic samples using carbon furnace atomic absorption spectrometry.

Author(s) -

Ilene Sussman,

Leslie C. MacGregor,

Barry R. Masters,

F M Matschinsky

Publication year - 1988

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/36.3.3343507

Subject(s) - atomic absorption spectroscopy , chemistry , mass spectrometry , absorption (acoustics) , analytical chemistry (journal) , carbon fibers , resolution (logic) , microdissection , chromatography , materials science , biochemistry , physics , quantum mechanics , artificial intelligence , composite number , computer science , composite material , gene

Carbon furnace atomic absorption spectrometry was used to measure the Na and K content of freeze-dried microscopic tissue samples. This method was sufficiently sensitive to measure pmol amounts of Na and K from tissue weighing 10-60 ng. Within the spatial resolution of the microdissection procedure, ion diffusion that might occur during cryosectioning, freeze-drying, and dissection of the tissue did not seem to be a problem. Data obtained with this methodology were in agreement with previously reported values of the Na and K content of various tissues, thus supporting the usefulness of this quantitative histochemical technique.

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