Immunohistochemical antigen demonstration in plastic-embedded lymphoid tissue. | Zendy

Harry van Goor | Zendy; G. Harms | Zendy; Peter O. Gerrits | Zendy; F. G. M. Kroese | Zendy; S. Poppema | Zendy; Joris Grond | Zendy

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Immunohistochemical antigen demonstration in plastic-embedded lymphoid tissue.

Author(s) -

Harry van Goor,

G. Harms,

Peter O. Gerrits,

F. G. M. Kroese,

S. Poppema,

Joris Grond

Publication year - 1988

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/36.1.3275710

Subject(s) - paraformaldehyde , immunohistochemistry , antigen , antigenicity , chemistry , antigen retrieval , monoclonal antibody , spleen , microbiology and biotechnology , methacrylate , antibody , biology , immunology , copolymer , polymer , organic chemistry

We describe a method for post-embedding immunohistochemical demonstration of a wide range of antigens in glycol methacrylate-embedded tissue. Rat spleen and thymus tissues were fixed by immersion in fixatives containing different concentrations of paraformaldehyde, washed in sucrose phosphate buffer, dehydrated in acetone, infiltrated in a glycol methacrylate mixture in which the commonly used softener 2-butoxyethanol was replaced by butaandiol monoacrylate, and embedded. Trypsin was used to re-expose the masked antigenicity. Excellent results were obtained with a panel of monoclonal antibodies (MoAbs) directed against T-cells, B-cells, Ia-positive cells, macrophages, follicular dendritic cells, and leucocyte common antigen-bearing cells. The method described combines exact localization of antigens with optimal tissue morphology.

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