Immunohistochemical localization of acetylcholine receptors at human endplates using a monoclonal antibody. | Zendy

L. M. E. Smit | Zendy; H. Veldman | Zendy; F.G.I. Jennekens | Zendy

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Immunohistochemical localization of acetylcholine receptors at human endplates using a monoclonal antibody.

Author(s) -

L. M. E. Smit,

H. Veldman,

F.G.I. Jennekens

Publication year - 1987

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/35.5.3549892

Subject(s) - immunoperoxidase , acetylcholine receptor , immunohistochemistry , monoclonal antibody , acetylcholine , paraformaldehyde , receptor , antibody , biology , pathology , chemistry , immunology , medicine , biochemistry , endocrinology

We describe a simple indirect immunohistochemical method for localization of acetylcholine receptors (AChR) in motor endplates at the light and electron microscopic level. This method involves the use of a monoclonal antibody directed against the main immunogenic region (MIR) of AChRs and is applicable to periodate-lysine-paraformaldehyde (PLP)-fixed tissue. We discuss the advantages of this method, as compared with the alpha-bungarotoxin-immunoperoxidase technique, and stress its value for diagnostic investigations of motor point biopsies from patients with neuromuscular transmission disorders.

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