Immunolocalization of glyceraldehyde-3-phosphate dehydrogenase, hexokinase, and carboxypeptidase Y in yeast cells at the ultrastructural level. | Zendy

Evert van Tuinen | Zendy; Howard Riezman | Zendy

Open Access

Immunolocalization of glyceraldehyde-3-phosphate dehydrogenase, hexokinase, and carboxypeptidase Y in yeast cells at the ultrastructural level.

Author(s) -

Evert van Tuinen,

Howard Riezman

Publication year - 1987

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/35.3.3546482

Subject(s) - immunoelectron microscopy , hexokinase , biochemistry , autolysis (biology) , glyceraldehyde 3 phosphate dehydrogenase , cytoplasm , cell fractionation , dehydrogenase , yeast , carboxypeptidase , glyceraldehyde , microbiology and biotechnology , biology , aldolase a , chemistry , enzyme , glycolysis , antibody , immunology

We have developed a simple and effective method to embed whole yeast cells in Lowicryl resins with excellent ultrastructural and antigenic preservation. Using affinity-purified antibodies eluted from electrophoretically separated proteins transferred to nitrocellulose, we have shown by immunoelectron microscopy that two glycolytic enzymes, glyceraldehyde-3-phosphate dehydrogenase and hexokinase, are present in the cytoplasm and the nucleus. Carboxypeptidase Y is localized in the yeast vacuole. These results agree with earlier localization studies based on subcellular fractionation.

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