Simultaneous visualization of myeloperoxidase reactivity and immunogold labeling by backscattered electron imaging with the scanning electron microscope. | Zendy

Davide Soligo | Zendy; Etienne P. de Harven | Zendy

Open Access

Simultaneous visualization of myeloperoxidase reactivity and immunogold labeling by backscattered electron imaging with the scanning electron microscope.

Author(s) -

Davide Soligo,

Etienne P. de Harven

Publication year - 1987

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/35.2.3025295

Subject(s) - immunogold labelling , electron microscope , scanning electron microscope , myeloperoxidase , cell , characterization (materials science) , scanning confocal electron microscopy , electron tomography , materials science , chemistry , biophysics , pathology , scanning transmission electron microscopy , optics , biology , physics , nanotechnology , biochemistry , immunology , medicine , inflammation

We describe a method to label circulating human granulocytes with an immunogold marker and then incubate them for demonstration of myeloperoxidase activity. The cells were observed in the backscattered electron imaging (BEI) mode of the scanning electron microscope. This permits simultaneous visualization of cell surface morphology, the immunological surface marker, and the cytochemical reactivity of each individual cell. Enhanced identification of the various cell types and more precise characterization of cell surface features in the different steps of leukocyte differentiation are expected to result from application of this technique.

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