Polymyxin B binding sites in Escherichia coli as revealed by polymyxin B-gold labeling. | Zendy

Hiroyuki Morioka | Zendy; M. Tachibana | Zendy; M Machino | Zendy; Atsushi Suganuma | Zendy

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Polymyxin B binding sites in Escherichia coli as revealed by polymyxin B-gold labeling.

Author(s) -

Hiroyuki Morioka,

M. Tachibana,

M Machino,

Atsushi Suganuma

Publication year - 1987

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/35.2.3025293

Subject(s) - polymyxin b , polymyxin , escherichia coli , colloidal gold , chemistry , bovine serum albumin , bacterial outer membrane , binding site , microbiology and biotechnology , biochemistry , biology , antibiotics , nanotechnology , materials science , gene , nanoparticle

A complex of polymyxin B, bovine serum albumin, and colloidal gold was prepared and used for the ultrastructural localization of polymyxin B binding sites on thin sections of Epon-embedded Escherichia coli cells. Gold particles were found on the outer membrane of E. coli, which is consistent with reported biochemical findings. We concluded that gold labeling with polymyxin B is useful in localizing the binding sites of polymyxin.

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