Differential immunolabeling for electron microscopy of diverse peptidergic neurons. | Zendy

Masahiko Maegawa | Zendy; Setsuji Hisano | Zendy; Yoshihiro Tsuruo | Zendy; Shinsuke Katoh | Zendy; Jun Nakanishi | Zendy; Mika ChikamoriAoyama | Zendy; Shigeo Daikoku | Zendy

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Differential immunolabeling for electron microscopy of diverse peptidergic neurons.

Author(s) -

Masahiko Maegawa,

Setsuji Hisano,

Yoshihiro Tsuruo,

Shinsuke Katoh,

Jun Nakanishi,

Mika ChikamoriAoyama,

Shigeo Daikoku

Publication year - 1987

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/35.2.2878952

Subject(s) - immunolabeling , vibratome , vasoactive intestinal peptide , suprachiasmatic nucleus , somatostatin , ultrastructure , chemistry , synaptogenesis , biophysics , microbiology and biotechnology , immunocytochemistry , biology , medicine , endocrinology , neuroscience , hypothalamus , neuropeptide , biochemistry , anatomy , immunohistochemistry , receptor

We describe a simple and reliable method for differential immunolabeling of pre- and post-synaptic signal peptides at the ultrastructural level. Hypothalamic tissues of rats, including the suprachiasmatic nucleus, were cut on a Vibratome. Visualization of the immunolabeling of somatostatin (SRIH) and vasoactive intestinal polypeptide (VIP) was performed with avidin-biotin-peroxidase-diaminobenzidine (DAB). The end product of the DAB to VIP was further silver-intensified in a physical processing using silver nitrate, and the silver grains were finally substituted for gold. DAB-labeled SRIH fibers synapse on gold-labeled VIP perikarya and dendrites in the suprachiasmatic nucleus.

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