Open AccessImproved preservation of glycogen in unfixed cyanobacteria embedded at -82 degrees C in nanoplast.
Author(s) -
D. Frōsch,
Christel Westphal,
Herbert Böhme
Publication year - 1987
Publication title -
journal of histochemistry and cytochemistry/the journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/35.1.3098832
Subject(s) - glycogen , cyanobacteria , staining , ultrastructure , melamine , chemistry , biochemistry , nuclear chemistry , botany , chromatography , biology , bacteria , organic chemistry , paleontology , genetics
A technique is presented for embedding unfixed cells at -82 degrees C in Nanoplast, a water-soluble melamine resin. By use of this procedure, improved ultrastructural preservation is demonstrated in cyanobacteria. Sections treated in this way are suitable for histochemical techniques such as Thiéry's silver staining method for glycogen.