Detection of polymorphic rat MHC antigen determinants in cryostat sections. A method for selecting monoclonal alloantibodies. | Zendy

Birte Steiniger | Zendy; J Klempnauer | Zendy; E. Günther | Zendy; K Wonigeit | Zendy

Open Access

Detection of polymorphic rat MHC antigen determinants in cryostat sections. A method for selecting monoclonal alloantibodies.

Author(s) -

Birte Steiniger,

J Klempnauer,

E. Günther,

K Wonigeit

Publication year - 1986

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/34.8.2426336

Subject(s) - monoclonal antibody , antigen , immunohistochemistry , antibody , monoclonal , microbiology and biotechnology , frozen section procedure , chemistry , immunology , biology , pathology , medicine

An immunohistological method for detection of rat MHC antigens by unconjugated primary alloantibodies is described. The procedure is based on secondary reagents specific for an allotypic marker of rat kappa light chains and makes possible differentiation between specifically bound alloantibody and the ubiquitously distributed interstitial immunoglobulin in the section. This technique can be used to pre-select monoclonal antibodies to be coupled to biotin or enzymes for histological application.

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