Electron microscopic localization of glutamate dehydrogenase in rat liver mitochondria by an immunogold procedure and monoclonal and polyclonal antibodies. | Zendy

Erwin Knecht | Zendy; A Martínez-Ramón | Zendy; Santiago Grisolı́a | Zendy

Open Access

Electron microscopic localization of glutamate dehydrogenase in rat liver mitochondria by an immunogold procedure and monoclonal and polyclonal antibodies.

Author(s) -

Erwin Knecht,

A Martínez-Ramón,

Santiago Grisolı́a

Publication year - 1986

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/34.7.3519755

Subject(s) - immunogold labelling , polyclonal antibodies , glutamate dehydrogenase , monoclonal antibody , immunocytochemistry , microbiology and biotechnology , mitochondrion , intracellular , biology , glutamate receptor , chemistry , antibody , biophysics , biochemistry , receptor , immunology , endocrinology

Glutamate dehydrogenase (GDH) was localized in rat liver by indirect electron microscopic immunogold, using different sizes of gold particles and monoclonal and polyclonal antibodies. Using the protein A-gold technique in double immunocytochemical experiments, both antibodies, at their optimal dilutions, gave similar results. A novel assessment of the distribution of GDH was made by measurements of the number of gold particles per square micrometer of cross-sectional images of individual mitochondria. The data indicate intracellular homogeneity among mitochondria in individual parenchymal cells. The enzyme is almost absent in non-parenchymal cells. Finally, GDH was found mainly in association with the mitochondrial inner membrane.

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