Specific detection of neuronal cell bodies: in situ hybridization with a biotin-labeled neurofilament cDNA probe. | Zendy

P. Liesi | Zendy; JeanPierre Julien | Zendy; Pekka Vilja | Zendy; Frank Grosveld | Zendy; Leena Rechardt | Zendy

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Specific detection of neuronal cell bodies: in situ hybridization with a biotin-labeled neurofilament cDNA probe.

Author(s) -

P. Liesi,

JeanPierre Julien,

Pekka Vilja,

Frank Grosveld,

Leena Rechardt

Publication year - 1986

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/34.7.3458811

Subject(s) - neurofilament , biotinylation , in situ hybridization , complementary dna , microbiology and biotechnology , neurite , biotin , immunocytochemistry , biology , silver stain , hybridization probe , in situ , chemistry , messenger rna , immunohistochemistry , biochemistry , dna , gene , in vitro , organic chemistry , immunology , endocrinology

We have used a biotinylated, 300-nucleotide cDNA probe which encodes the 68,000 MW neurofilament protein to detect neurofilament-specific mRNA in situ. The neurofilament message specifically demonstrates the neuronal cell bodies, in contrast to the usual antibody staining which detects their neurites. The hybridization is detected only in neuronal structures. Consequently, detection of the biotinylated neurofilament DNA probe by silver-intensified streptavidin-gold can be specifically used to identify neuronal cell bodies.

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