Open AccessImmunolocalization of band 3 protein in normal and cystic fibrosis skin.
Author(s) -
Debra J. HazenMartin,
Gary R. Pasternack,
Samuel S. Spicer,
Donald A. Sens
Publication year - 1986
Publication title -
journal of histochemistry and cytochemistry/the journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/34.6.3517151
Subject(s) - cystic fibrosis , sweat gland , sweat , chloride channel , chemistry , duct (anatomy) , membrane , chloride , immunohistochemistry , band 3 , pathology , eccrine sweat gland , endocrinology , anatomy , biology , medicine , biophysics , membrane protein , biochemistry , organic chemistry
Current evidence indicates that the defect in cystic fibrosis (CF) involves chloride transport in various epithelial cells. The sweat gland, one site of altered chloride transport in CF, was examined immunocytochemically for localization of a chloride-channel membrane protein, designated band 3 protein. Immunoreactivity was observed in sweat duct cell membranes of both normal and CF samples, whereas secretory coil regions were entirely unreactive. No difference was observed in the pattern or intensity of immunoreactivity between the two groups at the light microscopic (LM) level of resolution.