An improved method for tyrosine hydroxylase immunolabeling of catecholamine cells in whole mounted rat retina. | Zendy

Claudine VersauxBotteri | Zendy; J Nguyen-Legros | Zendy

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An improved method for tyrosine hydroxylase immunolabeling of catecholamine cells in whole mounted rat retina.

Author(s) -

Claudine VersauxBotteri,

J Nguyen-Legros

Publication year - 1986

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/34.6.2871070

Subject(s) - tyrosine hydroxylase , retina , immunolabeling , antiserum , penetration (warfare) , incubation , antibody , tyrosine , microbiology and biotechnology , catecholamine , chemistry , biology , endocrinology , immunohistochemistry , biochemistry , enzyme , immunology , neuroscience , operations research , engineering

We developed a rapid and efficient method to achieve good penetration of anti-tyrosine hydroxylase (TH) antiserum into whole mounted rat retinas. The retinas were defatted before incubation in the primary antibody. This operation was thought to increase membrane permeability, facilitating penetration of antibodies. As a result, all TH-positive cells in the entire retina were labeled. Thus, it becomes possible to study the morphology, the distribution, and the counting of TH immunoreactive cells in whole retinas.

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