Open AccessMurine endodermal cytokeratins Endo A and Endo B are localized in the same intermediate filament.
Author(s) -
W E Howe,
F. George Klier,
Robert G. Oshima
Publication year - 1986
Publication title -
journal of histochemistry and cytochemistry/the journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/34.6.2422254
Subject(s) - immunoelectron microscopy , intermediate filament , protein filament , electron microscope , cytoskeleton , vimentin , cytoplasm , chemistry , intracellular , biophysics , biology , microbiology and biotechnology , antibody , cell , immunohistochemistry , biochemistry , physics , immunology , optics
The intracellular distribution of extra-embryonic endodermal, cytoskeletal proteins A (Endo A) and B (Endo B) was investigated by double-label immunofluorescent microscopy and double-label immunoelectron microscopy. In parietal endodermal cells, the immunofluorescent distribution of Endo B was always coincident with that of Endo A and could be distinguished from vimentin, particularly at the periphery of the cell. At the electron microscopic level, antibodies against both Endo A and Endo B recognized both bundles and individual intermediate filaments. Double-label immunoelectron microscopy was achieved by use of two sizes of colloidal gold particles (5 nm and 20 nm) that were stabilized with secondary antibodies. These results show that Endo A and B are found in the same intermediate filament and probably co-polymerize to form such structures.