Alpha-fodrin in the adrenal gland: localization by immunoelectron microscopy. | Zendy

O.K. Langley | Zendy; Dominique Perrin | Zendy; Dominique Aunis | Zendy

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Alpha-fodrin in the adrenal gland: localization by immunoelectron microscopy.

Author(s) -

O.K. Langley,

Dominique Perrin,

Dominique Aunis

Publication year - 1986

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/34.4.3512700

Subject(s) - spectrin , immunoelectron microscopy , cytoplasm , cytoskeleton , chromaffin cell , subcellular localization , microbiology and biotechnology , ultrastructure , adrenal gland , biology , adrenal medulla , adrenal cortex , chemistry , antibody , cell , endocrinology , anatomy , biochemistry , immunology , catecholamine

Localization of fodrin, the brain equivalent of spectrin (a protein constituent of the erythrocyte membrane cytoskeleton), was investigated at the ultrastructural level in rat adrenal gland. By use of an affinity purified antibody directed against the alpha-fodrin subunit, all chromaffin cells, cortical cells, nerve fibers, and their surrounding Schwann cells were found to be labeled close to the cytoplasmic side of their plasma membranes. The labeling appeared more intense for chromaffin cells, and secretory granules and mitochondria were frequently found to be associated with the zone containing alpha-fodrin in these cells. The immunostained zone was estimated to extend 230 +/- 70 nm into the cytoplasm. This localization is discussed in terms of what is known of the properties of spectrin, and possible roles of the molecule in the chromaffin cell are suggested.

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