Microimmunoassay permits determination of concentrations in immunohistochemistry controls. | Zendy

D. Goldstein | Zendy; Mark M. Davis | Zendy; Robert K. Naviaux | Zendy; T L Dailey | Zendy; Thomas M. Ulbright | Zendy

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Microimmunoassay permits determination of concentrations in immunohistochemistry controls.

Author(s) -

D. Goldstein,

Mark M. Davis,

Robert K. Naviaux,

T L Dailey,

Thomas M. Ulbright

Publication year - 1986

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/34.4.2419398

Subject(s) - polyclonal antibodies , antiserum , immunohistochemistry , monoclonal antibody , antibody , amylase , value (mathematics) , antigen , microbiology and biotechnology , chemistry , enzyme , computer science , biology , biochemistry , immunology , machine learning

A competition enzyme-linked immunosorbant assay (CELIA) can be used to determine the amount of antigen needed to immunoabsorb an antiserum. This is demonstrated using both polyclonal and monoclonal antibodies against human amylase. This method is of especial value when the tissue is particularly difficult to obtain, and especially valuable when CELIA has already been completed.

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