Combined technique of immunohistochemistry and autoradiography for the simultaneous detection of steroid hormone receptors and their ligand. | Zendy

JeanMarie Gasc | Zendy; Bruce W. Ennis | Zendy; E.E. Baulieu | Zendy; Walter E. Stumpf | Zendy

Open Access

Combined technique of immunohistochemistry and autoradiography for the simultaneous detection of steroid hormone receptors and their ligand.

Author(s) -

JeanMarie Gasc,

Bruce W. Ennis,

E.E. Baulieu,

Walter E. Stumpf

Publication year - 1986

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/34.11.3772080

Subject(s) - immunohistochemistry , receptor , steroid , ligand (biochemistry) , steroid hormone , hormone , progestin , chemistry , antibody , progesterone receptor , nuclear receptor , antigen , biology , microbiology and biotechnology , medicine , biochemistry , immunology , cancer , gene , estrogen receptor , breast cancer , transcription factor

A combined technique of immunohistochemistry and autoradiography was applied to detect progesterone target cells in tissue sections. The radioactively labeled progestin [3H]-Organon 2058 and the progesterone receptor, revealed by antibodies to the receptor molecule, were localized simultaneously in identical cells on the same tissue section. Technical details that make possible combined detection of the nuclear antigen, present in very small amounts, simultaneously with its steroid ligand are described.

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