
Localization of eosinophil cationic protein, major basic protein, and eosinophil peroxidase in human eosinophils by immunoelectron microscopic technique.
Author(s) -
Arne Egesten,
J. Alumets,
Claes von Mecklenburg,
M Palmegren,
Inge Olsson
Publication year - 1986
Publication title -
journal of histochemistry and cytochemistry/the journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/34.11.3772075
Subject(s) - eosinophil peroxidase , eosinophil cationic protein , major basic protein , eosinophil granule proteins , eosinophil , immunoelectron microscopy , degranulation , granule (geology) , chemistry , granulocyte , microbiology and biotechnology , immunology , biology , biochemistry , immunohistochemistry , paleontology , receptor , asthma
An immunoelectron microscopic technique using protein A-gold as a specific marker was used for precise intracellular localization of eosinophil granule proteins. Eosinophils from healthy individuals were isolated in metrizamide gradients. Eosinophil cationic protein (ECP) and eosinophil peroxidase (EPO) were clearly located in the matrix of the large crystalloid-containing granules. In addition, ECP was probably present in the small granules of eosinophils. Major basic protein (MBP) was present in the crystalloid structure of specific granules. This method can be applied in studies of eosinophil degranulation to trace the release of biological effector molecules.