Ultrastructural autometallography: a method for silver amplification of catalytic metals. | Zendy

Gorm Danscher | Zendy; J. O. Rytter Nørgaard | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Ultrastructural autometallography: a method for silver amplification of catalytic metals.

Author(s) -

Gorm Danscher,

J. O. Rytter Nørgaard

Publication year - 1985

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/33.7.4008918

Subject(s) - uranyl acetate , photographic emulsion , emulsion , mercury (programming language) , silver bromide , chemistry , bromide , gelatin , colloid , particle (ecology) , electron microscope , materials science , inorganic chemistry , silver halide , optics , biochemistry , organic chemistry , geology , physics , programming language , oceanography , computer science

The autometallographic technique involves application of a silver bromide-containing emulsion on the surface of ultrathin sections placed on grids that are subsequently exposed to a photographic developer. In tissue sections from animals treated intravitally with gold, silver, or mercury compounds, accumulations of the metals are visualized by autometallography and can be used for quantitative studies. After amplification, sections can be stained with lead citrate and uranyl acetate. Using autometallography, particles of colloidal gold dispersed in a film of gelatin showed a time-dependent growth and were gradually amplified up to 3.5-fold after 15 min of development. Hence the method may prove useful tracing colloidal gold particles in sections with low particle density, and be a powerful tool for revealing metals in biological tissues.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research