Adenosine deaminase complexing proteins are localized in exocrine glands of the rabbit.
Author(s) -
William P. Schrader,
Charles A. West
Publication year - 1985
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/33.6.2582028
Subject(s) - serous fluid , bone canaliculus , staining , immunoperoxidase , exocrine gland , antibody , chemistry , biochemistry , adenosine deaminase , biology , microbiology and biotechnology , adenosine , monoclonal antibody , anatomy , secretion , genetics , immunology
Adenosine deaminase complexing proteins have been localized in four exocrine glands of the rabbit by immunoperoxidase staining employing affinity-purified goat anti-rabbit complexing protein immunoglobulin as the primary antibody. In pancreatic acinar cells and in serous cells of Brunner glands (duodenal glands), staining was concentrated in granular appearing deposits between the nucleus and cell apex. Bile canaliculi, components of the exocrine liver, were also positive for complexing protein. In submaxillary glands, staining was localized in serous demilunes and striated ducts. In each instance staining was blocked by preincubating the primary antibody with complexing protein purified from rabbit kidney.
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