A procedure for light and electron microscopic intracellular immunolocalization of collagen and fibronectin in rat liver. | Zendy

Bruno Clément | Zendy; Maryvonne Rissel | Zendy; S Peyrol | Zendy; Y Mazurier | Zendy; J.A. Grimaud | Zendy; A. Guillouzo | Zendy

Open Access

A procedure for light and electron microscopic intracellular immunolocalization of collagen and fibronectin in rat liver.

Author(s) -

Bruno Clément,

Maryvonne Rissel,

S Peyrol,

Y Mazurier,

J.A. Grimaud,

A. Guillouzo

Publication year - 1985

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/33.5.3886779

Subject(s) - fixative , fibronectin , golgi apparatus , intracellular , endoplasmic reticulum , immunoperoxidase , paraformaldehyde , microbiology and biotechnology , extracellular , extracellular matrix , chemistry , connective tissue , biology , biochemistry , biophysics , pathology , immunology , cytoplasm , medicine , antibody , organic chemistry , monoclonal antibody

Experimental conditions have been designed that permit both extracellular and intracellular immunolocalization of various collagen types and fibronectin in rat liver. The procedure involves paraformaldehyde fixation by perfusion of the organ, use of saponin as a membrane permeabilizing agent, and visualization of the matrix components by indirect immunoperoxidase. Intracellular demonstration of collagens was particularly sensitive to the composition of the fixative and the duration of fixation. Hepatocytes contained fibronectin and types I and IV collagen, whereas fat-storing and endothelial cells evidenced type III collagen in addition. All the components were specifically located in the endoplasmic reticulum and/or the Golgi apparatus.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research